Fagus Antibody Services

Recombinant Rat CD6/TP120 Protein (His Tag)(Active)

£363.20£884.00 excluding VAT

SKU FAS-030241-P Category Tag

T-cell differentiation antigen CD6, also known as TP120 and CD6, is a single-pass type I membrane protein which contains three SRCR domains. CD6 / TP120 is a cell surface glycoprotein expressed primarily on T cells, it may function as a costimulatory molecule and may play a role in autoreactive immune responses. CD6 / TP120 is expressed by thymocytes, mature T-cells, a subset of B-cells known as B-1 cells, and by some cells in the brain. CD6 ligand termed CD166 (previously known as activated leukocyte cell adhesion molecule, ALCAM ) has been identified and shown to be expressed on activated T cells, B cells, thymic epithelium, keratinocytes, and in rheumatoid arthritis synovial tissue. CD6 / TP120 binds to activated leukocyte cell adhesion molecule ( CD166 ), and is considered as a costimulatory molecule involved in lymphocyte activation and thymocyte development. CD6 / TP120 partially associates with the TCR / CD3 complex and colocalizes with it at the center of the mature immunological synapse (IS) on T lymphocytes. During thymic development CD6-dependent signals may contribute both to thymocyte survival, and to the overall functional avidity of selection in both man and mouse.

Size

100µg

Active Protein

Active protein

Activity

1. Immobilized rat CD6-His at 10 μg/mL (100 μL/well) can bind rat ALCAM-Fc3, The EC50 of rat ALCAM-Fc3 is 19.5-45.5 ng/ml. 2. Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells.When 8 x 104 cells/well are added to ratCD6-His coated plates (10μg/mL, 100 μL/well), approximately 39.2% will adhere after 60 minutes at 37℃.

Protein Construction

A DNA sequence encoding the rat CD6 (Q812A4) (Met1-Asn396) was expressed with a polyhistidine tag at the C-terminus.

Sequence

Met1-Asn396

Fusion Tag

C-His

Accession

Q812A4

Species

Rat

Expressed Host

HEK293 Cells

Shipping

This product is provided as lyophilized powder which is shipped with ice packs.

Purity

> 95 % as determined by reducing SDS-PAGE.

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Stability and Storage

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Mol Mass

42.5 kDa

AP Mol Mass

64-70 kDa

Formulation

Lyophilized from sterile PBS, pH 7.4

Research Areas
Reconstitution

Please refer to the printed manual for detailed information.

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