Fagus Antibody Services

Recombinant Rat EphA3 Protein (His Tag)(Active)

£684.00£968.00 excluding VAT

SKU FAS-030158-P Category Tag

EPHA3 gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. EPHA3 gene encodes a protein that binds ephrin-A ligands. EPHA3 is involved in the retinotectal mapping of neurons. It may also control the segregation but not the guidance of motor and sensory axons during neuromuscular circuit development.Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Size

200µg

Active Protein

Active protein

Activity

Measured by its binding ability in a functional ELISA.Immobilized rat EPHA3-His at 10 μg/ml (100 μl /well) can bind rat EFNA5-Fc, The EC50 of ratEFNA5-Fc is 9-20ng/ml.

Protein Construction

A DNA sequence encoding the rat EPHA3 (EDL75897.1) (Met1-His541) was expressed with a polyhistidine tag at the C-terminus.

Sequence

Met1-His541

Fusion Tag

C-His

Accession

EDL75897.1

Species

Rat

Expressed Host

HEK293 Cells

Shipping

This product is provided as lyophilized powder which is shipped with ice packs.

Purity

> 90 % as determined by reducing SDS-PAGE.

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Stability and Storage

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Mol Mass

61.2 kDa

AP Mol Mass

70.5 kDa

Formulation

Lyophilized from sterile PBS, pH 7.4

Research Areas
Reconstitution

Please refer to the printed manual for detailed information.

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