Fagus Antibody Services

Recombinant Rat TNF-alpha/TNFA Protein (Active)

£224.80£884.00 excluding VAT

SKU FAS-030443-P Category Tag

Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD120a; p55/60) and TNF-R2 (TNF receptor type 2; CD120b; p75/80), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.


20µg, 20µg

Active Protein

Active protein


Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of metabolic inhibitor actinomycin D. The ED50 for this effect is typically 20-80 pg/ml.

Protein Construction

A DNA sequence encoding the mature form of rat TNFα (P16599) (Leu 80-Leu 235) was expressed and purified, with an initial Met at the N-terminus.


Leu 80-Leu 235

Fusion Tag




Expressed Host



This product is provided as lyophilized powder which is shipped with ice packs.


> 93 % as determined by reducing SDS-PAGE.


Please contact us for more information.

Stability and Storage

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Mol Mass

17.4 kDa

AP Mol Mass

15 kDa


Lyophilized from sterile PBS, pH 7.4

Research Areas

Please refer to the printed manual for detailed information.

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