Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD120a; p55/60) and TNF-R2 (TNF receptor type 2; CD120b; p75/80), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.
Recombinant Rat TNF-alpha/TNFA Protein (Active)
£224.80 – £884.00 excluding VAT
| Size | 20µg, 20µg |
|---|---|
| Active Protein | Active protein |
| Activity | Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of metabolic inhibitor actinomycin D. The ED50 for this effect is typically 20-80 pg/ml. |
| Protein Construction | A DNA sequence encoding the mature form of rat TNFα (P16599) (Leu 80-Leu 235) was expressed and purified, with an initial Met at the N-terminus. |
| Sequence | Leu 80-Leu 235 |
| Fusion Tag | |
| Accession | P16599 |
| Species | Rat |
| Expressed Host | E.coli |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Purity | > 93 % as determined by reducing SDS-PAGE. |
| Endotoxin | Please contact us for more information. |
| Stability and Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Mol Mass | 17.4 kDa |
| AP Mol Mass | 15 kDa |
| Formulation | Lyophilized from sterile PBS, pH 7.4 |
| Research Areas | |
| Reconstitution | Please refer to the printed manual for detailed information. |
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