Fagus Antibody Services

Recombinant Rhesus macaque B7-H4/VTCN1 Protein (His Tag)

£73.60£166.40 excluding VAT

SKU FAS-050083-P Category

B7 Homolog 4 (B7-H4) is glycosylated member of the B7 family of immune costimulatory proteins.It is widely expressed, including in kidney, liver, lung, pancreas, placenta, prostate, spleen, testis and thymus. B7-H4 negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, plays an important role, together with regulatory T-cells (Treg), in the suppression of tumor-associated antigen-specific T-cell immunity. It also involved in promoting epithelial cell transformation.

Size

10µg, 50µg

Active Protein

Active protein

Activity

Immobilized Human GFRA1-His(Cat: PKSH033670) at 0.5μg/ml(100 μl/well) can bind Human GDNF. The ED50 of GDNF is 14.73ug/ml.

Protein Construction

Recombinant Rhesus macaque B7-H4 is produced by our Mammalian expression system and the target gene encoding Phe29-Ala258 is expressed with a 6His tag at the C-terminus.

Sequence

Phe29-Ala258

Fusion Tag

C-His

Accession

F7B770

Species

Rhesus macaque

Expressed Host

Human Cells

Shipping

This product is provided as lyophilized powder which is shipped with ice packs.

Purity

> 95 % as determined by reducing SDS-PAGE.

Endotoxin

< 1.0 EU per μg as determined by the LAL method.

Stability and Storage

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Mol Mass

26.4 kDa

AP Mol Mass

40-60 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

Research Areas
Reconstitution

Please refer to the printed manual for detailed information.

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