POLR3F encoded by this gene is one of more than a dozen subunits forming eukaryotic RNA polymerase III (RNA Pol III), which transcribes 5S ribosomal RNA and tRNA genes. This protein has been shown to bind both TFIIIB90 and TBP, two subunits of RNA polymerase III transcription initiation factor IIIB (TFIIIB). Unlike most of the other RNA Pol III subunits, the encoded protein is unique to this polymerase. Alternative splicing results in multiple transcript variants. POLR3F (RNA Polymerase III Subunit F) is a Protein Coding gene. Among its related pathways are Pyrimidine metabolism (KEGG) and RNA Polymerase III Transcription Initiation.