DHVD3(1,25-Dihydroxyvitamin D3) ELISA Kit

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with DHVD3. During the reaction, DHVD3 in samples or Standard competes with a fixed amount of DHVD3 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to DHVD3. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The concentration of DHVD3 in the samples is then determined by comparing the OD of the samples to the standard curve.