The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1; Sia, sialic acid; O-ac Sia, O-acetylated sialic acid. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Recombinant MERS-CoV S-trimer Protein (R751S, C-6His)(Active)
£304.00 – £5,875.00 excluding VAT
| Size | 50µg, 500µg |
|---|---|
| Active Protein | Active protein |
| Activity | Immobilized MERS-CoV S-trimer Protein (R751S)-His(PKSV030287) at 5μg/ml (100 μl/well) can bind Human CD26-Fc(PKSH033696), The ED50 of Human CD26-Fc(PKSH033696) is 27.16 ng/ml. |
| Protein Construction | Recombinant MERS-CoV S-trimer Protein is produced by our Mammalian expression system and the target gene encoding Tyr18-Lys1294(R751S) is expressed with a 6His tag at the C-terminus. |
| Sequence | Tyr18-Lys1294(R751S) |
| Fusion Tag | C-6His |
| Accession | AFS88936.1 |
| Species | MERS |
| Expressed Host | Human Cells |
| Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at<-20°C. |
| Purity | > 95 % as determined by reducing SDS-PAGE. > 90 % as determined by reducing SEC-HPLC. |
| Endotoxin | Please contact us for more information. |
| Stability and Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Mol Mass | 145kDa |
| AP Mol Mass | 170-220kDa |
| Formulation | Supplied as a 0.2 μm filtered solution of PBS, pH 7.4 |
| Research Areas | Virus related Protein |
| Reconstitution | Not Applicable |
