Fagus Antibody Services

Recombinant MERS-CoV S-trimer Protein (R751S, C-6His)(Active)

£304.00£5,875.00 excluding VAT

The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1; Sia, sialic acid; O-ac Sia, O-acetylated sialic acid. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Size

50µg, 500µg

Active Protein

Active protein

Activity

Immobilized  MERS-CoV S-trimer Protein (R751S)-His(PKSV030287) at 5μg/ml (100 μl/well) can bind Human CD26-Fc(PKSH033696), The ED50 of Human CD26-Fc(PKSH033696) is 27.16 ng/ml.

Protein Construction

Recombinant MERS-CoV S-trimer Protein is produced by our Mammalian expression system and the target gene encoding Tyr18-Lys1294(R751S) is expressed with a 6His tag at the C-terminus.

Sequence

Tyr18-Lys1294(R751S)

Fusion Tag

C-6His

Accession

AFS88936.1

Species

MERS

Expressed Host

Human Cells

Shipping

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at<-20°C.

Purity

> 95 % as determined by reducing SDS-PAGE. > 90 % as determined by reducing SEC-HPLC.

Endotoxin

Please contact us for more information.

Stability and Storage

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Mol Mass

145kDa    

AP Mol Mass

170-220kDa

Formulation

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4

Research Areas

Virus related Protein

Reconstitution

Not Applicable

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