Fagus Antibody Services

Recombinant SARS-CoV-2 S Protein RBD (C-6His) V2(Active)

£208.00£1,950.00 excluding VAT

The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. Most notable is severe acute respiratory syndrome (SARS). The severe acute respiratory syndrome-coronavirus (SARS-CoV) spike (S) glycoprotein alone can mediate the membrane fusion required for virus entry and cell fusion. It is also a major immunogen and a target for entry inhibitors. It’s been reported that 2019-nCoV can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Size

50µg, 500µg

Active Protein

Active protein

Activity

Immobilized 2019-nCoV S Protein RBD-His V2 (PKSV030308) at 5μg/ml (100 μl/well) can bind Human ACE-2-Fc(PKSR030492).The ED50 of Recombinant Human ACE-2-Fc(PKSR030492) is 417 ng/ml;Immobilized 2019-nCoV S Protein RBD-His V2 (PKSV030308) at 5μg/ml (100 μl/well) can bind Anti-2019-nCoV S1 mAb (5D9).The ED50 of Anti-2019-nCoV S1 mAb (5D9) is 117 ng/ml.

Protein Construction

Recombinant 2019-nCoV S protein RBD Protein is produced by our Mammalian expression system and the target gene encoding Arg319-Phe541 is expressed with a 6His tag at the C-terminus.

Sequence

Arg319-Phe541

Fusion Tag

C-6His

Accession

QHD43416.1

Species

SARS-CoV-2

Expressed Host

Human Cells

Shipping

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at<-20°C.

Purity

> 95 % as determined by reducing SDS-PAGE.

Endotoxin

Please contact us for more information.

Stability and Storage

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Mol Mass

25.9kDa  

AP Mol Mass

35kDa

Formulation

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.

Research Areas

Virus related Protein

Reconstitution

Not Applicable

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