This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with 15d-PGJ2. During the reaction, 15d-PGJ2 in samples or Standard competes with a fixed amount of 15d-PGJ2 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to 15d-PGJ2. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The concentration of 15d-PGJ2 in the samples is then determined by comparing the OD of the samples to the standard curve.
15d-PGJ2(15-Deoxy-Delta12,14-prostaglandin J2) ELISA Kit
£396.00 excluding VAT
| Size | 96T |
|---|---|
| Specificity | This kit recognizes 15d-PGJ2 in samples. No significant cross-reactivity or interference between 15d-PGJ2 and analogues was observed. |
| Application | This ELISA kit applies to the in vitro quantitative determination of 15d-PGJ2 concentrations in Serum, plasma and other biological fluids. |
| Research Areas | Signal transduction |
| Target Species | Universal |
| Assay Type | Competitive |
| Sensitivity | 1.88ng/mL |
| Detection range | 3.13~200ng/mL |
| Sample type | Serum, plasma and other biological fluids |
| Assay length | 2.5h |
| Storage | An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received. |
| Citation Link | https://bpspubs.onlinelibrary.wiley.com/doi/abs/10.1111/bph.14886, https://pubmed.ncbi.nlm.nih.gov/32694760/ |
