This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with VD3. During the reaction, VD3 in samples or Standard competes with a fixed amount of VD3 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to VD3. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The concentration of VD3 in the samples is then determined by comparing the OD of the samples to the standard curve.
VD3(Vitamin D3) ELISA Kit
£396.00 excluding VAT
| Size | 96T |
|---|---|
| Specificity | This kit recognizes VD3 in samples. No significant cross-reactivity or interference between VD3 and analogues was observed. |
| Application | This ELISA kit applies to the in vitro quantitative determination of VD3 concentrations in Serum, plasma and other biological fluids. |
| Research Areas | Signal transduction, Metabolism |
| Target Species | Universal |
| Assay Type | Competitive |
| Sensitivity | 0.94ng/mL |
| Detection range | 1.56~100ng/mL |
| Sample type | Serum, plasma and other biological fluids |
| Assay length | 2.5h |
| Storage | An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received. |
| Citation Link |
