This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with 3-NT. During the reaction, 3-NT in samples or Standard competes with a fixed amount of 3-NT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to 3-NT. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The concentration of 3-NT in the samples is then determined by comparing the OD of the samples to the standard curve.
3-NT(3-Nitrotyrosine) ELISA Kit
£396.00 excluding VAT
Size | 96T |
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Specificity | This kit recognizes 3-NT in samples. No significant cross-reactivity or interference between 3-NT and analogues was observed. |
Application | This ELISA kit applies to the in vitro quantitative determination of 3-NT concentrations in Serum, plasma and other biological fluids. |
Research Areas | Signal transduction |
Target Species | Universal |
Assay Type | Competitive |
Sensitivity | 0.94ng/mL |
Detection range | 1.56~100ng/mL |
Sample type | Serum, plasma and other biological fluids |
Assay length | 2.5h |
Storage | An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received. |
Citation Link | https://pubmed.ncbi.nlm.nih.gov/32148655/, https://www.sciencedirect.com/science/article/pii/S0003986118306684, https://pubmed.ncbi.nlm.nih.gov/32594586/, http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0174542, https://link.springer.com/article/10.1007/s12011-018-1537-1, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6066271/, http://www.njcponline.com/article.asp?issn=1119-3077;year=2019;volume=22;issue=2;spage=174;epage=180;aulast=Orimadegun, https://pubmed.ncbi.nlm.nih.gov/32474541/ |