Platelet endothelial cell adhesion molecule (PECAM-1, CD31) is a type I transmembrane glycoprotein adhesion molecule in the immunoglobulin superfamily. PECAM-1 is concentrated at cell junctions and is required for transendothelial migration (TEM). The extracellular domain (ECD) of PECAM-1 has ten potential N-linked glycosylation sites and six C2-type Ig-like domains, the first of which is critical for adhesion and extravasation. The cytoplasmic domain contains immunoregulatory tyrosine-based inhibitory and switch motifs (ITIM, ITSM) that mediate both inhibition and activation via phosphotyrosine-mediated engagement of SH2-containing signaling molecules. Expression is restricted to cells involved in circulation, especially endothelial cells, platelets, monocytes, neutrophils and lymphocyte subsets. PECAM-1 participates with other adhesion molecules in some functions, but is the critical molecule for TEM. Homotypic PECAM-1 adhesion in trans, combined with cycling of PECAM-1 to and from surface-connected endothelial cell vesicles, leads leukocytes across endothelial tight junctions.
Recombinant Mouse PECAM-1 is produced by our Mammalian expression system and the target gene encoding Glu18-Lys590 is expressed with a 6His tag at the C-terminus.
This product is provided as lyophilized powder which is shipped with ice packs.
> 95 % as determined by reducing SDS-PAGE.
< 1.0 EU per μg as determined by the LAL method.
|Stability and Storage||
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
|AP Mol Mass||
Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.
Please refer to the printed manual for detailed information.