Fagus Antibody Services

Recombinant Mouse CX3CL1/Fractalkine Protein (His Tag)

£93.60£237.60 excluding VAT

Fractalkine(CX3CL1) is a single-pass type I membrane protein and belongs to the intercrine delta family. It consists of an extracellular NH2-terminal domain, a mucin-like stalk, a transmembrane α helix, and a short cytoplasmic tail. CX3CL1 exists in two forms: as a membrane-anchored or as a shed 80-95K glycoprotein. Soluble CX3CL1 is generated by limited proteolysis on the cell surface, and a disintegrin and metallopeptidase 10 (ADAM10) and ADAM17/tumor necrosis factor-α-converting enzyme (ADAM17/TACE) participate in this shedding. It has been suggested that ADAM10 acts in the constitutive shedding, and ADAM17 acts in response to cell activation. The protein may play a role in regulating leukocyte adhesion and migration processes at the endothelium.

Size

10µg, 50µg

Active Protein
Activity
Protein Construction

Recombinant Mouse C-X3-C motif chemokine 1 is produced by our Mammalian expression system and the target gene encoding Gln25-Arg337 is expressed with a 6His tag at the C-terminus.

Sequence

Gln25-Arg337

Fusion Tag

C-6His

Accession

O35188

Species

Mouse

Expressed Host

Human Cells

Shipping

This product is provided as lyophilized powder which is shipped with ice packs.

Purity

> 95 % as determined by reducing SDS-PAGE.

Endotoxin

< 1.0 EU per μg as determined by the LAL method.

Stability and Storage

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.

Mol Mass

34.3 kDa

AP Mol Mass

58-60 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH7.4.

Research Areas
Reconstitution

Please refer to the printed manual for detailed information.

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